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with a range of substrate analogs. The reaction mechanism is of fascination since NagA from bacterial sources is found with either 1 or 2 divalent metal ions during the active web site.
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needs to be appreciably diverse since the H×H motif through the conclusion of β-stand one is replaced by Q×N and so it is very likely that this enzyme can bind only a single divalent cation within the Lively web site.
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Web page certain mutants of NagA were created in an effort to confirm the roles of distinct residues in substrate recognition and catalytic operate. The metal information and the kinetic parameters for your mutants of NagA made for this investigation are offered in Table four.
There's no proof to suggest that Glu-131 performs any job in catalysis besides to help you coordinate The one divalent cation. The enzyme is fee constrained with the cleavage in the amide bond.